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Microbiology

Semi-conservative DNA Replication

December 31, 2024 Sushil Humagain 0

Introduction DNA is only one type of genetic information that is carried by humans and other living things. The bases of the DNA known as […]

The Glyoxylate Cycle (Steps involved, Significance and Regulation)

June 27, 2021 Sushil Humagain 0

In plants, certain invertebrates and some microorganisms (including E. coli and yeast), acetate can serve both as an energy-rich fuel and as a source of […]

Regulation of gene expression of Lac Operon in E. coli

June 11, 2021 Sushil Humagain 0

Regulation of gene expression involves two steps; transcription and translation to form a complete protein or enzyme. Some enzymes are required under certain conditions but […]

Tollen’s test or Silver mirror test: Principle, Requirements, Procedure, Result Interpretation and Limitations

June 8, 2021 Sushil Humagain 0

Tollen’s test is a chemical test used to distinguish reducing sugars from non-reducing sugars. It is also known as silver mirror test since free silver […]

Antibody mediated immune response (AMI) and its effector mechanism

June 7, 2021 Sushil Humagain 0

Antibody mediated immune response (AMI) and its effector functions Antibody-mediated immune response is the host defense mechanism that is mediated by antibodies present in the […]

Five major classes of Antibodies and their Biological Activities

June 6, 2021 Sushil Humagain 0

Heavy-chain sequence analysis of an immunoglobulin molecule revealed five basic sequence patterns. These five basic sequences correspond to five different heavy-chain constant (C) regions named […]

Differences between 70S and 80S ribosomes

June 4, 2021 Sushil Humagain 0

S.N. Characteristics 70S Ribosomes 80S Ribosomes 1. Occurrence They occur in prokaryotic cells and in the mitochondria and plastids of eukaryotic cells. They occur only […]

Western blotting: Principle, Steps involved and Applications

June 3, 2021 Sushil Humagain 0

Western blotting technique is used for the separation and identification of a specific protein in a complex mixture of proteins based on their molecular weight. […]

Optochin Test: Principle, Requirements, Procedure, Result Interpretation and Limitations

June 1, 2021 Sushil Humagain 0

Principle: Optochin test is used to determine the effect of Optochin (ethylhydrocupreine hydrochloride) on an organism. Optochin is a chemical used as an antibiotic that […]

Classification of Streptococcus

June 1, 2021 Sushil Humagain 0

The genus Streptococcus includes a large number of species of Gram-positive, catalase negative cocci. They are non-motile and non-spore forming. They are arranged in chains […]

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