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Laboratory tests

Tollen’s test or Silver mirror test: Principle, Requirements, Procedure, Result Interpretation and Limitations

June 8, 2021 Sushil Humagain 0

Tollen’s test is a chemical test used to distinguish reducing sugars from non-reducing sugars. It is also known as silver mirror test since free silver […]

Western blotting: Principle, Steps involved and Applications

June 3, 2021 Sushil Humagain 0

Western blotting technique is used for the separation and identification of a specific protein in a complex mixture of proteins based on their molecular weight. […]

Optochin Test: Principle, Requirements, Procedure, Result Interpretation and Limitations

June 1, 2021 Sushil Humagain 0

Principle: Optochin test is used to determine the effect of Optochin (ethylhydrocupreine hydrochloride) on an organism. Optochin is a chemical used as an antibiotic that […]

Bacitracin Test or Bacitracin Susceptibility Test (Principle, Procedure, Requirements, Result Interpretation and  Limitations)

May 31, 2021 Sushil Humagain 0

Bacitracin test is one of the antimicrobial disc tests used to determine the susceptibility of bacteria to bacitracin. It is used for distinguishing group A […]

Benedict’s Test: Principle, Requirements, Procedure and Result Interpretation

November 11, 2020 Sushil Humagain 0

A sugar consists of an anomeric carbon to which a functional group is attached. If the anomeric carbon consists of aldehyde as a functional group, […]

Capsule staining: Principle, Requirements, Procedure and Microscopic Examination

October 8, 2020 Sushil Humagain 0

Capsule staining is a differential technique commonly used in light microscopy. A capsule is a gelatinous layer which lies immediately exterior to the cell wall […]

Acid-fast staining or Ziehl-Neelsen staining : Principle, Requirements, Procedure and Microscopic Examination

October 7, 2020 Sushil Humagain 0

Acid-fast staining is one of the differential staining techniques commonly used for light microscopic examination of bacteria. It differentiates acid-fast bacteria from non-acid fast bacteria. […]

Gram staining: Principle, Requirements, Procedure and Microscopic Examination

October 5, 2020 Sushil Humagain 0

Gram staining is a differential staining technique that is used for microscopic examination of bacteria. In differential staining, specimen is subjected to a series of […]

Staining: Purpose, Mechanism and Dyes/Stains used in light microscopy

October 4, 2020 Sushil Humagain 0

Purpose of staining: In bright field or light microscopy, background appears bright and the microorganisms appear dark as they absorb some of the light. However, […]

Fehling’s test (Principle, reagents and materials required, procedure and result interpretation)

November 14, 2019 Sushil Humagain 0

The Fehling test was developed in 1848 by Herrmann Fehling.  Like Benedict’s test, it is also a sensitive test for the detection of reducing sugars. […]

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