| S.N. | Hormones | Enzymes |
| 1. | Hormones may be amino acid derivatives, peptides, proteins or steroids in nature. | All enzymes are complex proteins. |
| 2. | They have low molecular weight. | They have high molecular weight. |
| 3. | They often readily diffuse through cell membranes. | They are not diffusible. |
| 4. | They are secreted by cells in endocrine glands at one site and are carried by blood to the target site. | They are secreted by cells in exocrine glands and may act in the cells themselves or pass via ducts to act in their target site. |
| 5. | They are often used up in their regulatory action. | They remain unaffected in the reaction they catalyze. |
| 6. | Hormone-controlled actions are not reversible. | Enzyme-controlled reactions are reversible. |
| 7. | Hormones either accelerate or reduce the rate of specific physiological process. | They normally speed up the biochemical reactions. |
| 8. | They are effective in low concentrations. | They also act in low concentrations, but the rate of enzyme-catalyzed reactions steadily increases with an increase in their concentration. |
| 9. | They may act either slowly or quickly. | They act quickly. |