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Bacteriology

Semi-conservative DNA Replication

December 31, 2024 Sushil Humagain 0

Introduction DNA is only one type of genetic information that is carried by humans and other living things. The bases of the DNA known as […]

Nitrogen fixation, its types and mechanism

April 3, 2023 Sushil Humagain 0

What is nitrogen fixation? The phenomenon of conversion of free nitrogen into nitrogenous salts to make it available for absorption by plants is called nitrogen […]

Classification of Streptococcus

June 1, 2021 Sushil Humagain 0

The genus Streptococcus includes a large number of species of Gram-positive, catalase negative cocci. They are non-motile and non-spore forming. They are arranged in chains […]

Bacterial endospore: Structure, Stages of Sporulation and Germination

May 28, 2021 Sushil Humagain 0

Spore is a resting or dormant cell which is metabolically inactive and is produced during unfavorable conditions like nutrition deficiency, extreme temperature and pH, presence […]

Bacterial biofilms, their formation and role in pathogenesis

May 7, 2021 Sushil Humagain 0

What is a biofilm? A biofilm is an aggregate of interactive bacteria attached to a solid surface or to each other and encased in an […]

Capsule staining: Principle, Requirements, Procedure and Microscopic Examination

October 8, 2020 Sushil Humagain 0

Capsule staining is a differential technique commonly used in light microscopy. A capsule is a gelatinous layer which lies immediately exterior to the cell wall […]

Acid-fast staining or Ziehl-Neelsen staining : Principle, Requirements, Procedure and Microscopic Examination

October 7, 2020 Sushil Humagain 0

Acid-fast staining is one of the differential staining techniques commonly used for light microscopic examination of bacteria. It differentiates acid-fast bacteria from non-acid fast bacteria. […]

Gram staining: Principle, Requirements, Procedure and Microscopic Examination

October 5, 2020 Sushil Humagain 0

Gram staining is a differential staining technique that is used for microscopic examination of bacteria. In differential staining, specimen is subjected to a series of […]

Staining: Purpose, Mechanism and Dyes/Stains used in light microscopy

October 4, 2020 Sushil Humagain 0

Purpose of staining: In bright field or light microscopy, background appears bright and the microorganisms appear dark as they absorb some of the light. However, […]

Normal microbial flora and their Roles in the Human Body

October 3, 2020 Sushil Humagain 0

The term “normal microbial flora” denotes the population of microorganisms that inhabit various body parts like skin and mucous membranes of healthy normal persons. Our […]

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